Biological nitrogen fixation and urea-N recovery in 'Coastcross-1' pasture treated with Azospirillum brasilense
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چکیده
منابع مشابه
Purification and binding analysis of the nitrogen fixation regulatory NifA protein from Azospirillum brasilense.
NifA protein activates transcription of nitrogen fixation operons by the alternative sigma 54 holoenzyme form of RNA polymerase. This protein binds to a well-defined upstream activator sequence (UAS) located at the -200/-100 position of nif promoters with the consensus motif TGT-N10-ACA. NifA of Azospirillum brasilense was purified in the form of a glutathione-S-transferase (GST)-NifA fusion pr...
متن کامل(Methyl)ammonium transport in the nitrogen-fixing bacterium Azospirillum brasilense.
An ammonium transporter of Azospirillum brasilense was characterized. In contrast to most previously reported putative prokaryotic NH4+ transporter genes, A. brasilense amtB is not part of an operon with glnB or glnZ which, in A. brasilense, encode nitrogen regulatory proteins PII and PZ, respectively. Sequence analysis predicts the presence of 12 transmembrane domains in the deduced AmtB prote...
متن کاملCharacterization of an Azospirillum brasilense Tn5 mutant with enhanced N(2) fixation: the effect of ORF280 on nifH expression.
Disruption of an open reading frame (ORF) of 840 bp (280 amino acids; ORF280) in an Azospirillum brasilense Tn5 mutant resulted in a pleiotrophic phenotype. Besides an enhanced N(2)-fixing capacity and altered expression pattern of a nifH-gusA fusion, growth on the charged polar amino acids glutamate and arginine was severely affected. ORF280, similar to previously identified ORFs present in Br...
متن کاملOccurrence of Azospirillum brasilense in soils amended with swine manure
Restriction analysis of amplified 16S rDNA from bacterial strains isolated from soil was used to evaluate the effects of swine manure and urea fertilisation on indigenous populations of the plant growth promoting bacterium, Azospirillum brasilense, in corncultivated experimental plots. Several operational taxonomic units (OTUs) were found among the isolates. One of them, identified as A. brasil...
متن کاملBiological Nitrogen Fixation with Emphasis on Legumes
1. Nitrogen-Fixing Organisms 2. Importance of Nitrogen Fixation 2.1. Inputs to the Ecosystems 2.2. Methods to Measure Nitrogen Fixation 3. The Rhizobium–Legume Symbiosis 3.1. The Rhizobia 3.2. Establishment of the Symbiosis 3.3. Functioning of the Root Nodule 4. Evolution and Ecology 5. Applications 5.1. Agronomy and Forestry 5.2. Biotechnology 6. Future Prospects Glossary Bibliography Biograph...
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ژورنال
عنوان ژورنال: Pesquisa Agropecuária Brasileira
سال: 2020
ISSN: 1678-3921,0100-204X
DOI: 10.1590/s1678-3921.pab2020.v55.01242